[vc_row css=”.vc_custom_1459506798851{margin-bottom: 100px !important;}”][vc_column offset=”vc_col-lg-9 vc_col-md-9″][vc_single_image image=”2161″ img_size=”stm-image-1110×550-croped” css=”.vc_custom_1577790280752{margin-bottom: 45px !important;}”][vc_custom_heading text=”Molecular Cloning” font_container=”tag:h4|text_align:left|color:%23222222″ use_theme_fonts=”yes” css=”.vc_custom_1577790783557{margin-bottom: 31px !important;}”][vc_tta_accordion shape=”square” c_position=”right” active_section=”1″ css=”.vc_custom_1574145661399{margin-bottom: 34px !important;}”][vc_tta_section tab_id=”1452765420119-d4dfaf69-4fae” title=”Training in Recombine DNA Technology Duration: 15 days “][vc_row_inner][vc_column_inner width=”3/4”][vc_column_text]
Preparation of nucleic acids
- Plant Genomic DNA extraction
- Plasmid DNA extraction
- Purification of gDNA & plasmid DNA
[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_empty_space height=”70px”][vc_btn title=”Apply” style=”classic” shape=”round” color=”violet” size=”xs” align=”center” css_animation=”bounceInRight” button_block=”true” link=”url:http%3A%2F%2Fcawordpressdemos.com%2Fca_2019_hybrinomics%2Fonline-registration%2F|||”][/vc_column_inner][/vc_row_inner][/vc_tta_section][vc_tta_section title=”Qualitative analysis of gDNA & plasmid DNA” tab_id=”1574146865006-b8f67bf9-f1d4″][vc_row_inner][vc_column_inner width=”3/4″][vc_column_text]Agarose gel electrophoresis. Spectrophotometer, Dot spot
Preparation of target gene fragment:
- PCR techniques
- Agarose gel electrophoresis
- Gel elution
[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_empty_space height=”70px”][vc_btn title=”Apply” style=”classic” shape=”round” color=”violet” size=”xs” align=”center” css_animation=”bounceInRight” button_block=”true” link=”url:%23|||”][/vc_column_inner][/vc_row_inner][/vc_tta_section][vc_tta_section title=”Preparation of plasmid DNA & gene fragment for cloning:” tab_id=”1574146864373-74aec471-8f15″][vc_row_inner][vc_column_inner width=”3/4″][vc_column_text]
- Restriction digestion
- Dot spot quantification
- Ligation
[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_empty_space height=”70px”][vc_btn title=”Apply” style=”classic” shape=”round” color=”violet” size=”xs” align=”center” css_animation=”bounceInRight” button_block=”true” link=”url:%23|||”][/vc_column_inner][/vc_row_inner][/vc_tta_section][vc_tta_section title=”Competent cell Preparation” tab_id=”1574146863557-49506da1-f1e4″][vc_row_inner][vc_column_inner width=”3/4″][vc_column_text]
- Preparation of nutrient broth
- Starter culture preparation
- Preparation of competent cells
- Storage of competent cells
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- Preparation of nutrient broth
- Starter culture preparation
- Preparation of competent cells
- Storage of competent cells
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